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Effects of 1-MCP and ethylene on expression of three CAD genes and lignification in stems of harvest

来源: 南亚热带作物研究所    更新: 2010-09-08    浏览: 9038    字体:

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Food Chemistry
Volume 123, Issue 1, 1 November 2010, Pages 32-40

doi:10.1016/j.foodchem.2010.03.122 | How to Cite or Link Using DOI
Copyright © 2010 Elsevier Ltd All rights reserved.
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Effects of 1-MCP and ethylene on expression of three CAD genes and lignification in stems of harvested Tsai Tai (Brassica chinensis)

Lubin Zhanga, b, Guang Wangb, Jinmei Changa, Jianshuang Liub, Jianghua Caib, Xiuwen Raob, Lijuan Zhangb, Juanjuan Zhongb, Jianghui Xiea and Shijiang Zhub, Corresponding Author Contact Information, E-mail The Corresponding Author

a Hainan Key Laboratory for Postharvest Physiology and Technology of Tropical Horticultural Products, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, Guangdong Province, China

b Guangdong Province Key Laboratory of Postharvest Physiology and Technology of Fruits and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong Province, China

Received 7 December 2009; 
revised 8 February 2010; 
accepted 30 March 2010. 
Available online 2 April 2010.

 
 

Abstract

Tsai Tai (Brassica chinensis) is produced mainly in China but also consumed overseas. The stems of Tsai Tai are subject to toughening due to lignification. Cinnamyl alcohol dehydrogenase (CAD) is a key enzyme involved in lignin biosynthesis. Three full-length CAD cDNAs were isolated, encoding proteins of 288, 323, and 323 amino acids, respectively. Sequence analysis showed that they share a highly conserved putative NAD/NADP(H)-binding site at the amino terminus. All the three BcCAD genes responded to 1-MCP and ethylene within 2 h. Ethylene up-regulated expression of BcCAD1-1 and BcCAD2, while 1-MCP down-regulated them. Ethylene increased yellow leaves and stem lignin, and 1-MCP decreased them. These results suggest ethylene is involved in lignin growth in Tsai Tai, and induced expression of BcCAD1-1 and BcCAD2 could contribute to lignification. They also suggest removal of ethylene and application of 1-MCP could extend shelf-life and knockout of certain CAD genes could produce lines that store well.

Keywords: Cinnamyl alcohol dehydrogenase; Ethylene; 1-MCP; Senescence; Lignification; Brassica chinensis

Article Outline

1.
Introduction
2.
Materials and methods
2.1. Plant material and treatment
2.2. Extraction of RNA and isolation of full-length cDNA of CAD genes
2.3. Sequence analysis
2.4. CAD gene expression analysis
2.5. Lignin content assay
2.6. Design and analysis
3.
Results
3.1. Isolation and analysis of Tsai Tai BcCAD cDNA sequences
3.2. Effect of 1-MCP and ethylene on expression of BcCAD genes during and after treatment
3.3. Effect of 1-MCP and ethylene treatment on leaf yellowing of Tsai Tai shoots
3.4. Effect of 1-MCP and ethylene treatment on lignin contents of Tsai Tai stems
4.
Discussion
4.1. BcCAD1-1, BcCAD1-2 and BcCAD2 sequence analysis
4.2. Patterns of response of BcCAD to duration of treatments
4.3. Role of regulation of BcCAD genes in senescence-related lignification
4.4. Potential application of inhibition of lignification in Tsai Tai industry
5.
Conclusion
Acknowledgements
References







Corresponding Author Contact InformationCorresponding author. Tel.: +86 20 38902512/13632355208; fax: +86 20 85288280.

Food Chemistry
Volume 123, Issue 1, 1 November 2010, Pages 32-40
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